Western blot shows lysates of MCF-7 human breast cancer cell line, SH-SY5Y human neuroblastoma cell line, human muscle tissue, and human placenta tissue. PVDF membrane was probed with 1ug/ml of Sheep Human Dystroglycan Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. Specific bands were detected for A-Dystroglycan at approximately 100-160kD and B-Dystroglycan at ~42-44kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Dystroglycan was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Sheep Human Dystroglycan Antigen Affinity-purified Polyclonal Antibody at 3ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to basement membrane. View our protocol for Chromogenic IHC Staining of Paraffinembedded Tissue Sections.
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